With Thiol-C3, a 3-carbon spacer arm separates the Thiol moiety and the oligonucleotide, thereby reducing steric interactions between the Thiol and the oligo itself. metabion offers Thiol-C3 in its standard DNA portfolio and the Thiol-C3 modified oligos will be supplied as disulfides. Customer should reduce the disulfide bonds by using TCEP, DTT or other reducing agents to generate a reduced thiol group. For a short protocol click on the following link.
Metabion offers Thiol-C3 modification in its DNA portfolio. Click here
|Specifications as a 3' modification|