7-deaza-dG can be inserted in an oligonucleotide to create an asymmetric base pair. The Tm of the 7-deaza-dG: C binding is 1 °C lower, compared to the natural G: C binding. Therefore, replacing native Gs with 7-deaza-dG in GC-rich sequences can partially solve problems arising from G or GC-rich regions, such as formation of secondary structure.
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|Extinction coefficient (260 nm)||12.6 mM-1cm-1|
F. Seela, and H. Driller. Alternating d(G-C)3 and d(C-G)3 hexanucleotides containing 7-deaza-2’-deoxyguanosine or 8-aza-7-deaza-2’-deoxyguanosine in place of dG. Nucleic Acids Res. (1989), 17: 901-910.