5-Bromo deoxycytosine (Bromo-dC) is a halogenated nucleotide, commonly used in X-ray crystallography to facilitate the determination of DNA structure. Additionally, they are photo-labile and therefore suitable for UV-crosslinking experiments to study protein-DNA complexes. 5-Br-dU was also used to create photo-aptamers able to crosslink with their target.
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|Extinction coefficient (260 nm)||3.1 mM-1cm-1|
W. Hendrickson, C. Ogata. Phase determination from multiwavelength anomalous diffraction measurements. Meth. Enzymol. (1997), 276: 494-523.