Dabcyl

Dabcyl is a dark quencher often used in dual-labelled probes. The 3’-terminus of Dabcyl consists of a free OH. When Dabcyl is inserted at the 3’-end of a primer/probe, the polymerase can theoretically elongate the primer/probe. However, the efficiency of this elongation is rather low.

 Quencher dabcyl

Metabion offers Dabcyl in its DNA and RNA dual-labelled probes portfolio. Click here

Dabcyl as 5' modification
MW as 5' 430.2 g/mol
Absorption range 380-550 nm
Absorption maximum 453 nm
Extinction coefficient (260nm) 11.1 mM-1cm-1
Dabcyl as 3' modification
MW 756 g/mol
Absorption range 380-550 nm
Absorption maximum 453 nm
Extinction coefficient (260nm) 11.1 mM-1cm-1
Dabcyl as an internal modification
MW 745 g/mol
Absorption range 380-550 nm
Absorption maximum 453 nm
Extinction coefficient (260nm) 20.4 mM-1cm-1

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