C3 spacer represents an effective way to block polymerase extension, when positioned at the 3’-end of an oligonucleotide. Additionally, it can prevent exonuclease-dependent oligo cleavage.
Metabion offers C3 spacer modification in its DNA and RNA portfolio. Click here
K.W. Cradic, J.E. Wells, L. Allen, K.E. Kruekeberg, R.J. Singh, and S.K.G. Grebe. Substitution of 3'-phosphate cap with a carbon-based blocker reduces the possibility of fluorescence resonance energy transfer probe failure in real-time PCR assays. Clinical Chemistry.(2004); 50:1080–1082.