We have a very efficient monoclonal antibody production service using mice immunized with antigens either provided by the customer or produced by us (recombinant proteins - synthetic peptides). On average 1-2 mg of protein are necessary for immunization and analysis. This service includes the generation of monoclonal antibodies through somatic fusion and the possibility to choose either 2 or 4 hybridoma clones after the first screening. We can provide both, the cell lines and the purified monoclonal ABs. Upon request, a cell banking service is available for the storage of the desired clones.
The development and production of monoclonal antibodies goes through different phases:
- Production of recombinant protein (if necessary)
Gene cloning
Protein expression
Protein purificaton
- Synthesis of peptide and conjugation (if necessary)
Synthesis of about 10 mg of peptide, purity > 70%
Conjugation of 3-4 mg of peptide to a carrier protein (as antigen)
Conjugation of 1-2 mg of peptide to a different carrier protein for ELISA screening
- Phase I (1.5 months)
Immunization of 3 BALB/c mice with the antigen
Setup of a procedure for quantitative titration of specific immunoglobulins in the sera of immunized animals. ELISA testing of the immune response and selection of the animals for subsequent spleen cell fusion.
- Phase II (2 months)
Splenectomy and fusion of splenocytes with myeloma cells
Selection of hybridomas by ELISA: freezing of the more positive mother clones
Delivered products: supernatants of mother clones (about 5 ml each)
- Phase III (1-1.5 months)
Cloning and subcloning of positive clones (2 or 4) by limiting dilution
Freezing of the hybridomas (2 aliquots/clone)
Viability, productivity and stability assays after thawing of one aliquot.
Delivered products: frozen hybridomas (3 cryo-vials of each selected sub-clone + 2 cryo-vials of each mother clone)
- Phase IV (1-1.5 months)
Production and purification of 0.5-2 mg of each monoclonal antibody in "cell culture"
Delivery time of a monoclonal antibody should be estimated at 7-10 months depending on the complexity of requested services. Close interaction with the customer at any step of production is of the essence!
Price scheme as follows:
| Monoclonal Antibodies (MABs) | |||
|---|---|---|---|
| Cat # | Description | Prices € | |
| MAB-2139 | Phase I | Immunization of 4 mice | 600,00 |
| MAB-2140 | Phase I | Immunization of 4 mice against a recombinant protein, including gene-cloning, protein expression and protein purification | 1.650,00 |
| MAB-2144 | Phase I | Immunization of 4 mice against 1 synthetic peptide (15 mer *), including synthesis and carrier conjugation (OVA for immunization and KLH for screening) | 1.150,00 |
| MAB-2141 | Phase II | Spleen fusion | 2.850,00 |
| MAB-2135 | Phase III Cloning and subcloning (2 clones ) | 2.000,00 | |
| MAB-2145 | Phase III | Handling and storing of mouse spleens (1 up to 4 spleens) for 3 months | 325,00 |
| MAB-2142 | Phase III | Cloning of each additional clone | 500,00 |
| MAB-2143 | Clone Storage / 6 months ( 3 vials per clone) | 125,00 | |