“ready-to-use/fully labeled” custom oligonucleotides
Making use of utmost flexibility spaces out standardization as far as feasibility and pricing schedules are concerned. Hence, we would like to invite you to send us your requests for so far “extraterrestrial” oligos indicating sequence and modification demands. We will check your inquiry and come back to you with solutions and/or proposals that will surprise you. Use our “discovery channel” and send your request to info@metabion.com.“ready-to-click-it-yourself” custom oligonucleotides
a) Order your alkyne-modified oligo by sending an email to oligo@mymetabion.com.
Make sure to include all relevant information like
- shipping address & contact information (person, phone, fax, email)
- billing address & contact information (person/customer ID, phone, fax, email, VAT #)
- quotation and/or PO # if any
- product specification including
- oligo name
- purification option - standard or HPLC
- synthesis scale/desired minimum final quantity delivered
- delivery form (dry or wet)
- sequence 5´-3´ and position of the Alkyne-modification according to the following instructions:
Use respective Modification shortcuts listed in the table below.
There are 2 ways to introduce Alkyne groups to the 5´end of the oligo:
1) Alxyl is sequence independent, adding an Alkyne group via a C6 (hexynyl)- linker to the phosphate of the 5´ nucleoside.
2) AldU and AldC carry a C8-Alkyne motif at the C5 position of the respective Pyrimidine structure of either dU (replacing dT) or dC. Hence, if there is a C or T at the 5´ end of the oligo sequence, Alkyne-activation can also be introduced through AldC or AldU (sequence dependent).
For internal Alkynylation either choose AldU or AldC to replace dT and/or dC within the oligo sequence.
For Alkyne-activation of the 3´ end again, there are two options:
1) Alrol is sequence independent, adding an Alkyne group via a serinol-linker to the 3´ OH of the 3´ nucleoside (3´OH-blocking).
2) AldU and AldC carry a C8-Alkyne motif at the C5 position of the respective Pyrimidine structure of either dU (replacing dT) or dC. Hence, if there is a C or T at the 3´ end of the oligo sequence, Alkyne-activation can also be introduced through AldC or AldU (sequence dependent) without blocking the 3´OH end.
Alkynylation site Labelling through … Modification shortcut 5' Hexynyl-PA
C8-Alkyne-dU-PA
C8-Alkyne-dC-PAAlxyl
AldU
AldCinternal C8-Alkyne-dU-PA
C8-Alkyne-dC-PAAldU
AldC3' 3'-Alkyne-Modifier Serinol CPG
C8-Alkyne-dU-PA
C8-Alkyne-dC-PAAlrol
AldU
AldC
b) Optionally select a suitable “click-partner” for your Alkyne modified oligonucleotide from our Azide-modifiers and additional “click”-reagents list. Download the respective order form (download pdf), fill it in, and fax it to +49 (0)89 899 363 11.
“modimerization” solutions
Select the individual products you need from the 3 sections
- Alkyne Nucleotides (download order form)
- Polymerase (download order form)
- Azide-Modifiers and Additional “Click” Reagents (download order form)
or choose one of metabion´s attractive
“Click” Packages (download order form)
and place your order either by
- sending an email to mi@mymetabion.com indicating all relevant information like
- shipping address & contact information (person, phone, fax, email)
- billing address & contact information (person/customer ID, phone fax, email, VAT #)
- article/catalogue # and quantity.
- sending the filled in “Click” order forms by fax to +49 (0)89 899 363 11.
or
1. Alkyne Nucleotides
“Click”-chemistry allows for simple, quick, and efficient post-synthetic modification at multiple positions of alkynylated DNA (e.g. for FISH, EMSA etc.). Moreover, alkynylated DNA itself can be used directly in various applications including nanotechnology.
| Alkyne Nucleotides | ||||
|---|---|---|---|---|
| Cat. No. | Product | Concentration | Amount | Price |
| mi-N3001S | C8-Alkyne-dCTP | 100 mM | 0,5 µmol | 115,00 |
| mi-N3001M | C8-Alkyne-dCTP | 100 mM | 2,5 µmol | 445,00 |
| mi-N3001L | C8-Alkyne-dCTP | 100 mM | 5,0 µmol | 650,00 |
| mi-N3002S | C8-Alkyne-dUTP | 100 mM | 0,5 µmol | 115,00 |
| mi-N3002M | C8-Alkyne-dUTP | 100 mM | 2,5 µmol | 445,00 |
| mi-N3002L | C8-Alkyne-dUTP | 100 mM | 5,0 µmol | 650,00 |
2. Polymerase
Alkyne nucleotides do not serve as substrate for conventional Taq. We recommend metabion´s proofreading Polymerase.
| Polymerases | |||
|---|---|---|---|
| Cat. No. | Product | Amount | Price |
| mi-E6022 | mi-Pfu Set | 250 u | 85,00 |
3. Azide-Modifiers and additional “Click” Reagents
Azide-activated molecules can be used to modify alkynylated DNA/RNA sequences and other alkynylated biomolecules.
| Fluorescent Azide-Modifier | |||
|---|---|---|---|
| Cat. No. | Product | Amount | Price |
| mi-C1001S | 6-FAM-Azide | 1 mg | 165,00 |
| mi-C1001M | 5 mg | 520,00 | |
| mi-C1004S | 5-FAM-Azide | 1 mg | 165,00 |
| mi-C1004M | 5 mg | 520,00 | |
| mi-C1005S | Fluoresceine-Azide (5/6-FAM) | 1 mg | 125,00 |
| mi-C1005M | 5 mg | 400,00 | |
| mi-C1006S | Chromeo™ 642-Azide * | 1 mg | 320,00 |
| mi-C1006M | 5 mg | 1350,00 | |
| mi-C1007S | Chromeo™ 546-Azide ** | 1 mg | 320,00 |
| mi-C1007M | 5 mg | 1350,00 | |
| mi-C1008S | 5-TAMRA-Azide | 1 mg | 190,00 |
| mi-C1008M | 5 mg | 650,00 | |
| mi-C1010S | Eterneon™-350/430 Azide | 1 mg | 55,00 |
| mi-C1010M | 5 mg | 210,00 | |
| mi-C1011S | Eterneon™-350/455 Azide | 1 mg | 55,00 |
| mi-C1011M | 5 mg | 210,00 | |
| mi-C1012S | Eterneon™-384/480 Azide | 1 mg | 55,00 |
| mi-C1012M | 5 mg | 210,00 | |
| mi-C1013S | Eterneon™-393/523 Azide | 1 mg | 55,00 |
| mi-C1013M | 5 mg | 210,00 | |
| mi-C1014S | Eterneon™-394/507 Azide | 1 mg | 55,00 |
| mi-C1014M | 5 mg | 210,00 | |
| mi-C1015S | Eterneon™-480/635 Azide | 1 mg | 60,00 |
| mi-C1015M | 5 mg | 230,00 | |
* equivalent to CY5, ** equivalent to CY3
| Non-Fluorescent Azide-Modifier | |||
|---|---|---|---|
| Cat. No. | Product | Amount | Price |
| mi-C1002S | Dabcyl-Azide | 1 mg | 145,00 |
| mi-C1002M | 5 mg | 490,00 | |
| mi-C1003S | Biotin-Azide | 1 mg | 135,00 |
| mi-C1003M | 5 mg | 460,00 | |
| Additional “Click” Reagents | |||
|---|---|---|---|
| Cat. No. | Product | Amount | Price |
| mi-C1101S | CuBr | 5 mg | 10,00 |
| mi-C1101M | 10 x 5 mg | 80,00 | |
| mi-C1102S | TBTA-Ligand | 5 mg | 24,00 |
| mi-C1102M | 10 mg | 39,00 | |
| mi-C1103S | DMSO/t–Butanol Solvent | 1,0 ml | 10,00 |
| mi-C1103M | 10 x 1,0 ml | 75,00 | |
All Prices in Euro, excluding tax and transport. For MSDS please visit our download section.
„Click“ Packages
| „Click“ PCR Packages | |||
|---|---|---|---|
| Cat. No. | Product | Content | Price |
| mi-CPCR1_S | Click PCR Package 1 S |
mi-Pfu Set, mi-E6022 dNTP Set, mi-N1005S C8-Alkyne-dCTP, mi-N3001S C8-Alkyne-dUTP, mi-N3002S |
350,00 |
| mi-CPCR1_L | Click PCR Package 1 L |
mi-Pfu Set, mi-E6022 dNTP Set, mi-N1005S C8-Alkyne-dCTP, mi-N3001L C8-Alkyne-dUTP, mi-N3002L |
1250,00 |
| mi-CPCR2_S | Click PCR Package 2 S |
Click PCR Package S + mi-PCR Purification Kit, mi-PP200 |
470,00 |
| mi-CPCR2_L | Click PCR Package 2 L |
Click PCR Package L + mi-PCR Purification Kit, mi-PP200 |
1350,00 |
| „Click“ Modifier Packages | |||
|---|---|---|---|
| Cat. No. | Product | Content | Price |
| mi-CMod_Basic | Click Basic Modifier Kit |
CuBr, mi-C1101S TBTA-Ligand, mi-C1102S DMSO/t–Butanol Solvent, mi-C1103S (2x) |
49,00 |
| mi-CMod_S-6FAM | Click Advanced Modifier Kit S-6FAM |
CuBr, mi-C1101S (2x) TBTA-Ligand, mi-C1102M DMSO/t–Butanol Solvent, mi-C1103S (4x) 6-FAM-Azide, mi-C1001S |
235,00 |
| mi-CMod_S-5FAM | Click Advanced Modifier Kit S-5FAM |
CuBr, mi-C1101S (2x) TBTA-Ligand, mi-C1102M DMSO/t–Butanol Solvent, mi-C1103S (4x) 5-FAM-Azide, mi-C1004S |
235,00 |
| mi-CMod_S-FLU | Click Advanced Modifier Kit S-FLU |
CuBr, mi-C1101S (2x) TBTA-Ligand, mi-C1102M DMSO/t–Butanol Solvent, mi-C1103S (4x) Fluoresceine-Azide, mi-C1005S |
200,00 |
| mi-CMod_S-BIO | Click Advanced Modifier Kit S-BIO |
CuBr, mi-C1101S (2x) TBTA-Ligand, mi-C1102M DMSO/t–Butanol Solvent, mi-C1103S (4x) Biotin-Azide, mi-C1003S |
210,00 |
Useful links
Protocol for PCR incorporation of Alkyne-dNTPs
http://www.organic-chemistry.org/namedreactions/click-chemistry.shtm
http://www.lbl.gov/Science-Articles/Archive/MSD-click-chemistry.html