Dual labelled probes - DLPs

metabion's dual labelled probes (DLPs) are optimized for use in real-time quantitative PCR. Moreover, our DLPs are produced according to technologically advanced synthesis and purification protocols, to ensure you that the eventual background noise will be reduced to an absolute minimum.

Our range of reporter-quencher combinations suit probe hydrolysis as well as probe hybridisation based assays. We believe this will cover your needs for single or multiplex real-time PCR applications.

We offer our DLPs in final yields in nmoles. With this, we introduce a transparent and best price-performance scheme for our broad selection of dual labelled probes as follows:

  • 5 scales based on delivered quantities tailored to your routine needs (see our Portfolio).

  • Length independent guaranteed (range) of delivered quantities in nmoles for probes from 6 to 40mers. Please note that OD260 values are a measure of total nucleotides´ optical density. Hence, neither purity nor amount of ordered substance are transparently reflected. For simplification and exemplification reasons look at the following:

    1 OD of the 20mer 5´CAT CGT ATT CGA TGC TAC GT 3´
    translates into approximately 5 nmol.

    1 OD of the 40mer 5´CAT CGT ATT CGA TGC TAC GT CAT CGT ATT CGA TGC TAC GT 3´
    translates into approximately 2.5 nmol.

    Therefore, a 1 OD guaranteed amount of delivered product can vary significantly, while metabion´s commitment to delivered yields in nmol for probes ranging from 6 to 40 nucleotides does not allow for ambiguity in terms of what you expect and pay for.

  • metabion´s routine dual labeled probe portfolio perfectly covers the spectrum of applicable excitation and detection wavelengths, with Reporter-Quencher combinations that are suitable for all commonly used Real Time PCR platforms. However, do not hesitate to ask for non-listed combinations including internally or multi-labeled oligos and probes. Contact us!

  • Our Prices includes HPLC (pop-up) purification as well as Mass-Check QC for our high-quality products.

It matters what you actually get for your money!

Any question? Visit our FAQs page or contact us!

Our Standard Dual-labelled fluorogenic probes Portfolio

5 yield ranges* based on length independent delivered quantities from 6-40 bases.

  • ≥ 5 < 10 nmol
  • ≥ 10 < 20 nmol
  • ≥ 20 < 30 nmol
  • ≥ 30 < 50 nmol
  • ≥ 50 < 70 nmol

* based on oligonucleotides of 6-40 bases in length; please note that HPLC purification and QC by Mass-Check is included

Reporter-Quencher combinations

3' Quencher
  TAMRA BHQ®-1 BHQ®-2 BBQ®-650 BHQ®-3 Eclipse® Dabcyl    
Abs range (nm) 470-560 480-580 550-650 550-750 620-730 390-625 380-550    
Abs max (nm) 544 535 579 650 672 522 453    
5' Reporter Possible combinations Abs (nm) Em (nm)
Atto 390         390 476
Atto 425       439 485
LC® CYAN500         450 500
6-Fam     495 520
Fluo     495 520
FITC     490 525
Atto 495        498 526
Tet     521 536
Atto 520        517 538
Joe       522 548
Yakima Yellow®       530 549
Hex   535 556
Atto Rho6G    533 557
Cy3         546 563
Tamra       564 579
Rox       576 601
Texas Red®         586 610
LC® Red 610         590 610
Atto Rho13          603 627
DY480XL           500 630
LC® Red 640         625 640
Atto Rho14          625 646
Cy5         646 662
Cy5.5       683 705
IRD®700       685 705

 

5' Quencher
  BHQ®-1 BHQ®-2 BBQ®-650 Eclipse® Dabcyl    
Abs range (nm) 480-580 550-650 550-750 390-625 380-550    
Abs max (nm) 535 579 650 522 453    
3' Reporter Possible combinations Abs (nm) Em (nm)
6-Fam   495 520
Fluo   495 520
Joe     522 548
Hex     535 556
Cy3         546 563
Rox     576 601
LC® Red 610     590 610
LC® Red 640       625 640
Cy5       646 662
Cy5.5       683 705

All our DLP probes are HPLC purified and mass-checked for best quality assurance!

DNA Oligo price list download PDF

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DNA Dual Labeled Probes
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Also available in our web order system (WOP)

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DNA Portfolio price list
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