LightCycler/HybProbes - LCPs

Introduced to the market in 1997, Light Cycler technology has become increasingly popular in various areas, from research to diagnostics.

The LightCycler® (LC®) hybridisation probe system is based on two oligonucleotide probes that hybridize, in a head-to-tail arrangement, to adjacent sequences of the target DNA (hybridisation assays). Each probe carries a different marker dye, the first one 3'Fluo, the second one either 5'LC®610, 5'LC®640, 5'LC®670 or 5'LC®705. Additionally, LC® Probes' 3'OH terminus is "capped" by a phosphate group to prevent polymerase from extending the primer during PCR reaction.

As the marker dyes are in close proximity, fluorescence resonance energy transfer (FRET) between them takes place which allows a specific and quantitative real-time detection of the amplification of a nucleic acid template. To avoid sterical problems between the 3' Fluo and 5' LC® Red end labels of the probes there should be a spacer of 1 to 5 nucleotides (4 to 25 Å distance) to separate the two probes from each other.

Metabion synthesizes LC® probes in an ISO-certified manufacturing facility using processes licensed by Roche. We offer LC®probes in 7 different scales at defined molar amounts.

LightCycler® probes - Fluorophores
Dye Abs (nm) Em (nm)
 LC® CYAN 500  450  500
 LC® Fluo  495  520
 LC® Red 610  590  610
 LC® Red 640  625  640
 LC® Red 670  650  670
 LC® Red 705 685  705
  • These fluorophores can be positioned internally and at the 5' or 3'-end, to produce dual or multiple labelled probes. To learn more click here
  • We offer LC® probes with a maximum length of 40 nucleotides in guaranteed delivered quantities between 1 and 30 nmol. Our service includes HPLC and Mass Check. Check our price list
  • For larger quantities, contact us!

 

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