RNA Longmers

With over 20 years of experience in oligo synthesis, metabion is now raising its standards even higher with our longly tried and trusted RNA Longmers!

 

Our offer in a nutshell:

✓ metabion Longmers are offered at a standard unmatched length of 140 nts

✓ HPLC-purified by default*

✓ delivered with complementary mass-spec trace documentation

✓  unmatched standard selection of modifications for RNA 81-140 Longmers

✓ our standard RNA modifications for RNA 60-80 Longmers

✓ available in dry, single tube format 

 

 * Please note that HPLC purification assures that the hightest possible percentage of our delivered RNA Longmers will be a full-length product. Many oligo providers claim that their coupling efficiency reaches 99.3%. This is currently a standard coupling efficiency and presents no guarantee in terms of delivered full-length product. With their standard 99.3% coupling efficiency, the crude synthesis-extract of oligonucleotides as long as 120-140 nucleotides will contain a quantity of full-length product not exceeding 25% of the ordered amount. Our known commitment and ability to deliver defined amounts of final product becomes evident when we deliver HPLC purified, 100% Mass-Checked RNA Longmers. As we like to say:

it matters what you actually get for your money!

 

If you intend to use RNA longmers for CRISPR-Cas applications, you might consider using Breaking-Cas* to design the guideRNA portion of your RNA longmer.

Any questions? Visit our FAQs page or contact us!

*Spanish National Centre for Biotechnology (CNB-CSIC)

Our standard custom RNA longmers portfolio features

  • 4 final yields based on length independent delivered quantities from 1-10 nmol for single stranded oligos from 60-140 nucleotides.
        • 1 nmol
        • 2 nmol
        • 5 nmol
        • 10 nmol
  • 100% HPLC purification* 

* Please note that HPLC purification assures that the hightest possible percentage of our delivered RNA Longmers is a full-length product. Many oligo providers claim that their coupling efficiency reaches 99.3%. This is currently a standard coupling efficiency and presents no guarantee in terms of delivered full-length product. With 99.3% coupling efficiency, the crude synthesis-extract of oligonucleotides as long as 120-140 nucleotides will contain a quantity of full-length product not exceeding 25% of the ordered amount. Our known commitment and ability to deliver defined amount of final product becomes evident with our choice of delivered HPLC purified, 100% Mass-Checked RNA Longmers.  

  • Quality Control by Mass-Check for best quality

ESI 100mer Fig.1 Authentic mass-Spec trace of a RNA 100 longmer

  • An extended standard selection of single modifications (5´, internal, 3´) for RNA longmers ranging from 81-140 bases.
  • For RNA oligos up to 80mers you can draw from our rather “unlimited” resources available for our standard RNA modification portfolio.
  • Delivery in single tubes, shipping condition dry.
  • Comprehensive and best value pricing scheme.

Fluorescent Modifications

TypePositionSpectrum Maxima
  5' Abs Em
Atto390 390 476
Atto425 439 485
6-Fam 495 520
Fluo 495 520
Tet 521 536
Joe 522 548
Yakima Yellow® 530 549
Hex 535 556
Cy3 546 563
Atto550 554 576
Tamra 564 579
Texas Red® 586 610
Atto 633 630 651
Cy5 646 662
Atto647N 646 664
Cy5.5 683 705

For more information, click on our price list

 

Non-Fluorescent Modifications

TypePosition
  5' 3' internal
Phosphate    
C6 Amino    
Biotin  ✓  
C6 Thiol  ✓    
C3 spacer  
Cholesteryl  
DNA
PTO
2' F  
2' OMe  ✓
7-deaza-G  
C6-Amino-U    
Biotin-TEG  

Any question? Visit our FAQs page or contact us!

 

RNA Longmers price list
download PDF
RNA Portfolio price list download PDF

Our service at a glance

  • You order your desired oligos with the possibility to choose between a seasoned selection of modifications.
  • For further assistance with your order, you can always rely on our top quality Customer Service via chat, telephone or email.
  • If you order within 18.00 (German time), we start processing your order on the same day.
  • Unmodified and single-modified RNA longmers will in average be shipped within 8-15 working days, depending on sequence length/complexity and modification level.
  • You will receive your oligos dry. The synthesis report delivered with the order will give you information on the amount of water or buffer to be added to get a 100µM solution.
  • RNA longmers can be ordered in distinct final yields.
  • All our RNA oligonucleotides are HPLC purified and checked for their quality with Mass spectrometry by default.

Useful Links

Breaking Cas Our page
Introduction to CRISPR-Cas  Learning Platform

Order Forms

RNA longmers Order  download xls
Also available in our web order system (WOP)

RNA Guidelines

Guidelines for Handling RNA download PDF

Credit Card Payment Form

Credit Card Payment Form download PDF

Price Lists

RNA Longmers price list
download PDF
RNA Portfolio price list download PDF