Click Chemistry

Since 2010, Click Chemistry became a hot topic in oligonucleotide labeling. Always striving to be at the forefront of technical improvements in order to serve our customers best, metabion has secured a worldwide license for employing this interesting chemistry on producing modified oligonucleotides.

Click chemistry is a versatile technique that can be used for the synthesis of a variety of conjugates. Virtually it can be applied to any biomolecules. With respect to oligonucleotide labeling, click chemistry allows for multiple labeling of polymerized DNA strands via incorporation of Alkyne-dNTPs (triphosphates) followed by post-synthetic click reactions at a so far unmet efficiency level.

Want to learn more about the concept of click chemistry? Visit our Learning Platform! Click here
Which applications are possible with click chemistry?

Extended means of (multiple) labeling of biomolecules such as Nucleic Acids (DNA/RNA), peptides, proteins, and antibodies will have a vigorous effect on the following areas (making no claim to be exhaustive):

  • Fluorescence In Situ Hybridization (FISH)
  • qPCR-Assays
  • DNA/RNA Microarrays
  • Bioconjugation to Nanoparticles
  • Fluorescence Microscopy
  • Electrophoretic Mobility Shift Assay (EMSA)
  • Fluorescence Activated Cell Sorting (FACS)
  • Flow Cytometry (FCM), Cell Tracking and Cell-based Assays
  • Fluorescence Correlation Spectroscopy (FCS)

What we offer

1. “ready-to-use/fully labeled” custom oligonucleotides

Having successfully introduced this additional labeling procedure for synthetic Nucleic Acids and other biomolecules into our custom synthesis operations, we are now able to offer significantly increased flexibility in terms of

  • multiple labeling of oligonucleotides (2 or more modifications)
  • producing modified oligonucleotides exceeding a critical sequence length.

Send us your requests to info@metabion.com. We will check your inquiry and come back to you with solutions and/or proposals that will surprise you.

2. “ready-to-click-it-yourself” custom oligonucleotides

Moreover, we can provide you with alkyne-modified oligonucleotides to be labeled at your end by “clicking” the desired “Azide-modification”. For more information, see our full list of Click-modifications below.
There are 2 ways to introduce Alkyne groups to the 5´end of the oligo:

  1. You can introduce an Alxyl moiety, which is sequence independent and allows the addition of an Alkyne group via a C6 (hexynyl)- linker to the phosphate of the 5´ nucleoside.
  2. If your oligo sequence carries a C or T at the 5´ end, you can introduce an Alkyne-activation site via replacing the C/T with AldC/ AldU, respectively (sequence dependent). AldU and AldC carry a C8-Alkyne motif at the C5 position of the respective Pyrimidine structure of either dU (replacing dT) or dC.

For internal Alkynylation, you can either choose AldU or AldC to replace dT and/or dC within the oligo sequence.

For Alkyne-activation of the 3´ end, again, there are two options:

  1. You can introduce an Alrol moiety, which is sequence independent and connects your oligo with an Alkyne group via a serinol-linker to at the 3´ OH of the 3´ nucleoside (thus producing a 3´OH-blocking).
  2. If your oligo sequence carries a C or T at the 3´ end, you can introduce an Alkyne-activation site via replacing the dC/ dT with AldC/ AldU, respectively (sequence dependent). AldU and AldC carry a C8-Alkyne motif at the C5 position of the respective Pyrimidine structure of either dU (replacing dT) or dC. This way, you can introduce a 3’ Alkyne-activation site without blocking the 3´OH end
Alkylation siteLabelling throughModification shortcut
5' Hexynyl-PA
C8-Alkyne-dU-PA
C8-Alkyne-dC-PA
Alxyl
AldU
AldC
internal C8-Alkyne-dU-PA
C8-Alkyne-dC-PA
AldU
AldC
3' 3'-Alkyne-Modifier Serinol CPG
C8-Alkyne-dU-PA
C8-Alkyne-dC-PA
Alrol
AldU
AldC

For detailed information about how to order, visit our FAQs page

3. “modimerization” solutions

In line with above´s “click it yourself” approach, we can also supply you with:

  • Alkyne Nucleotides (triphosphates) to be incorporated into DNA during polymerization reactions (PCR, reverse transcription, nick-translation, etc.). This prepares the produced DNA strands for post-synthetic “click” conjugations to azid-activated labels – a very elegant and efficient way of “modimerizing” DNA strands.
  • Click-chemistry efficient Polymerase (Modimerase), a proofreading polymerase, which efficiently incorporates alkyne-modified dNTPs. Alkyne nucleotides do not serve as substrate for conventional Taq.
  • Azide Modifiers and additional “click reagents”. Azide-activated molecules can be used to modify alkynylated DNA/RNA sequences and other alkynylated biomolecules.

For detailed information about how to order, visit our FAQs page

List of Click-modifications

 Cat. No.ProductConcentrationAmount
Alkyne Nucleotides mi-N3001S
mi-N3001M
mi-N3001L
C8-Alkyne-dCTP 100 mM 0,5 µmol
2,5 µmol
5,0 µmol
mi-N3002S
mi-N3002M
mi-N3002L
C8-Alkyne-dUTP 100 mM 0,5 µmol
2,5 µmol
5,0 µmol
Polymerases mi-E9000 mi-Modimerase - 200 u
Non Fluorescent Azide Modifiers mi-C1002S
mi-C1002M
Dabcyl-Azide - 1 mg
5 mg
mi-C1003S
mi-C1003M
Biotin-Azide - 1 mg
5 mg
Additional „Click“ Reagents mi-C1101S
mi-C1101M
CuBr - 5 mg
10 x 5 mg
mi-C1102S
mi-C1102M
TBTA-Ligand - 5 mg
10 mg
mi-C1103S
mi-C1103M
DMSO/t–Butanol Solvent - 1,0 ml
10 x 1,0 ml
Fluorescent Azide Modifiers mi-C1001S
mi-C1001M
6-FAM-Azide - 1 mg
5 mg
mi-C1005S
mi-C1005M
Fluo-Azide (5/6-FAM) - 1 mg
5 mg
mi-C1008S
mi-C1008M
5-TAMRA-Azide - 1 mg
5 mg
mi-C1011S
mi-C1011M
Eterneon™-350/455 Azide - 1 mg
5 mg
mi-C1013S
mi-C1013M
Eterneon™-393/523 Azide - 1 mg
5 mg
mi-C1014S
mi-C1014M
Eterneon™-394/507 Azide - 1 mg
5 mg

 

 Price Lists

DNA Oligos price list download PDF
RNA Oligos price list download PDF
Life Science Products price list download PDF

Price Lists

DNA Oligos price list download PDF
RNA Oligos price list download PDF
Life Science Products price list download PDF

How to order

Use our Web Order Portal: Order now

or

Email: please send an email to mi@mymetabion.com specifying your order as well as your shipping and billing address, and any other information which might be required like PO number, VAT number (foreign country EU countries, beside Germany) etc.

Downloads

Cat. No.ProductData SheetMSDS
mi-N3001 C8-Alkyne-dCTP download PDF download PDF
mi-N3002 C8-Alkyne-dUTP download PDF download PDF
mi-E9000 mi-Modimerase download PDF ---
mi-C1002 Dabcyl-Azide download PDF download PDF
mi-C1003 Biotin-Azide download PDF download PDF
mi-C1101 CuBr download PDF download PDF
mi-C1102 TBTA-Ligand download PDF download PDF
mi-C1103 DMSO/t–Butanol Solvent download PDF download PDF
mi-C1001 6-FAM-Azide download PDF download PDF
mi-C1005 Fluoresceine-Azide (5/6-FAM) download PDF download PDF
mi-C1008 5-TAMRA-Azide download PDF download PDF
mi-C1011 Eterneon™-350/455 Azide download PDF download PDF
mi-C1013 Eterneon™-393/523 Azide download PDF download PDF
mi-C1014 Eterneon™-394/507 Azide download PDF download PDF
  DMSO --- download PDF
  t–Butanol Solvent --- download PDF