Methods for synthesizing peptides are divided into two distinct categories: solution and solid phase. We use the solid-phase peptide synthesis method with Fmoc-chemistry. The peptide chains are assembled on a solid support initiating at the C-terminus and progressing towards the N-terminus, by repeating three basic steps: deprotection of the Fmoc alpha amino group, activation of the next protected amino acid as an active ester, and coupling. When the synthesis is complete, the peptide is cleaved from the support with concomitant removal of side-chain protecting groups. We normally use automated synthesis but for difficult or particular sequences, we also employ manual synthesis.
