The definition of the entire structure of the oligosaccharide moieties released from glycoproteins is achieved by determining the microheterogeneity of the different structures, the composition of the monosaccharides present, the branching pattern, the sequence of the antennae, and the possible occurrence of modifying groups, such as sulphate, phosphate, acetyl groups etc. Furthermore, the linkage positions of the individual sugars in the oligosaccharide chains and the glycosylation sites in the protein sequence are also determined.
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