Description
The mi-DNA/RNA precipitator is a fast and easy to use neutral polyacrylamide polymer solution specially synthesized to act as a very efficient carrier for precipitation of a very small quantity of nucleic acids with ethanol. It provides a high quantitatively recovery from the solution of which concentration is more than 20ng/ml of DNA (>100bps) or RNA (>120bs).
mi-DNA/RNA precipitator is time saving because an incubation at –20° or –80° is not required. Immediate centrifugation after the addition of ethanol is possible.
The mi-DNA/RNA Precipitator is enzymaticaly neutral and does not inhibit any enzyme reactions. The precipitate obtained using mi-DNA/RNA precipitator is readily solution in a buffer appropriate for following experiments. When ethanol is added, the mi-DNA/RNA Precipitator forms a clearly visible pellet by itself after the centrifugation.
Principle
The mi-DNA/RNA precipitator provides a neutral polyacrylamide polymer matrix for nucleic acids to interact and to coprecipitate after adding ethanol forming a clearly visible pellet at room temperature.
Advantages
Rapid precipitation (less than 10 min)
Easy protocol
No incubation at minus degrees necessary. It is immediately possible to centrifuge after adding ethanol.
Highly quantitative recovery even of small DNA/RNA quantities or concentrations (>20ng/ml of DNA (>100bp) or RNA (>120bp).
A clearly visible pellet is formed after centrifugation
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