Description
The mi-Gel Extraction Kit is a high performance simple to use product to recover pure DNA from all kind of agarose gels.
The isolation procedure can be performed in less than 10-20 minutes. It is not necessary to use low melt agarose to get high yields. The resulting DNA can be used for any downstream application.
Principle
The overall principle of DNA purification by silica-membrane technology is the specific and selective adsorption of DNA molecules to the silica-gel–membrane in the spin column. The cells are lysed during a short incubation with Proteinase K in the presence of chaotropic salt which immediately inactives all nucleases and enhances the binding of DNA. After the binding of the DNA in optimized high-salt buffers to the surface, all contaminants like primers, nucleotides, enzymes, mineral oil, salts, agarose, ethidium bromide, and other impurities from DNA samples, can be removed through specialized wash buffers. The purified DNA can be eluted by low-salt buffer or distilled water.
Advantages
Easy protocol
Rapid isolation in 10-20 min
High recovery rates : up to 95%
DNA size range 80 bp ~ 50 kb
Types of agarose : all
Types of gel buffers : all
Maximum gel band weight : 200 mg per Spin Column
Final volume of DNA : 30 µl
No need of organic solvent
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