Description
The mi-Tissue Genomic DNA Kit is used to quickly and efficiently isolate high molecular weight genomic DNA (50-100 kb) from tissue. This kit is based on the efficient release of genomic DNA by the special cell lysis buffer and rapid separation of genomic DNA from proteins, polysaccharides and lipids due to selective adsorption of the DNA to a silica membrane. The DNA isolated can be used for all molecular biology applications (RFLP, PCR, Southern blotting, etc). This method is fast, safe and simple for DNA preparation from different sources of samples.
Principle
The overall principle of DNA purification by silica-membrane technology is the specific and selective adsorption of DNA molecules to the silica-gel–membrane in the spin column. The cells are lysed during a short incubation with Proteinase K in the presence of chaotropic salt which immediately inactives all nucleases and enhances the binding of DNA. After the binding of the DNA in optimized high-salt buffers to the surface, all contaminants like primers, nucleotides, enzymes, mineral oil, salts, agarose, ethidium bromide, and other impurities from DNA samples, can be removed through specialized wash buffers. The purified DNA can be eluted by low-salt buffer or distilled water.
Advantages
Rapid isolation within 1 hour
Easy protocol
High efficiency: Yield range of 25-50 mg mammalian tissue : 10-20ug
Yield range of 106 Culture cells: 15-20ug
High reproducibility
All types of animal tissues and cell cultures
Maximum 100 mg wet weight or up to 20 mg dry weight (lyophilized) material.
High molecular weight genomic DNA size up to: 50-100 kb
The isolated DNA can be used for all molecular biology applications (RFLP, PCR, Southern blotting, etc).
Final volume of DNA : 50 µl
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