Description
The mi-Yeast Genomic DNA Kit is used to quickly and efficiently isolate high molecular weight genomic DNA (50-100kb) from yeast. This kit is based on the efficient release of genomic DNA by using a special cell lysis buffer. A rapid separation of genomic DNA from proteins, polysaccharides and lipids by selective adsorption of DNA to the silica membrane becomes possible. The isolated DNA can be used for all molecular biology applications (RFLP, PCR, Southern Blotting, etc). This method is fast, safe and simple for DNA preparation from different sources of samples.
Principle
The overall principle of DNA purification by silica-membrane technology is the specific and selective adsorption of DNA molecules to the silica-gel–membrane in the spin column. The cells are lysed during a short incubation with Proteinase K in the presence of chaotropic salt which immediately inactives all nucleases and enhances the binding of DNA. After the binding of the DNA in optimized high-salt buffers to the surface, all contaminants like primers, nucleotides, enzymes, mineral oil, salts, agarose, ethidium bromide, and other impurities from DNA samples, can be removed through specialized wash buffers. The purified DNA can be eluted by low-salt buffer or distilled water.
Advantages
Easy protocol
Rapid isolation (60-90min)
Highly efficiency
High reproducibility
High molecular weight genomic DNA size up to: 50-100 kb
The DNA isolated can be used for all molecular biology applications (RFLP, PCR, Southern Blotting, etc).
Final volume of DNA : 50 µl
Downloads