Multi-labelled custom probes

We are proud to provide our customers with top quality products, developed and produced using state-of-art technology. An excellent example is our synthesis service for custom FISH probes, which offers significantly increased flexibility in terms of

  • multiple labeling of custom FISH probes (2 or more modifications)
  • production of modified probes exceeding a critical sequence length.

This unbeatable offer is possible due to click chemistry, which allows for multiple labeling of polymerized DNA strands via incorporation of Alkyne-dNTPs (triphosphates) followed by post-synthetic click reactions at a so far unmatched efficiency level. Want to learn more about click chemistry? Click here

We offer our customers the opportunity to either:

  • Order “Ready-to-use/fully labeled” custom probes. Send us your requests to info@metabion.com. We will check your inquiry and come back to you with solutions and/or proposals that will surprise you.
  • Order “ready-to-click-it-yourself” custom probes

    We can provide you with alkyne-modified oligonucleotides to be labeled at your end by “clicking” the desired “Azide-modification”. Click here for our full list of Click-modifications.

    There are 2 ways to introduce Alkyne groups to the 5´end of the oligo:

    1. You can introduce an Alxyl moiety, which is sequence independent and allows the addition of an Alkyne group via a C6 (hexynyl)- linker to the phosphate of the 5´ nucleoside.
    2. If your oligo sequence carries a C or T at the 5´ end, you can introduce an Alkyne-activation site via replacing the C/T with AldC/ AldU, respectively (sequence dependent). AldU and AldC carry a C8-Alkyne motif at the C5 position of the respective Pyrimidine structure of either dU (replacing dT) or dC.

    For internal Alkynylation, you can either choose AldU or AldC to replace dT and/or dC within the oligo sequence.

    For Alkyne-activation of the 3´ end, again, there are two options:

    1. You can introduce an Alrol moiety, which is sequence independent and connects your oligo with an Alkyne group via a serinol-linker to at the 3´ OH of the 3´ nucleoside (thus producing a 3´OH-blocking).
    2. If your oligo sequence carries a C or T at the 3´ end, you can introduce an Alkyne-activation site via replacing the dC/ dT with AldC/ AldU, respectively (sequence dependent). AldU and AldC carry a C8-Alkyne motif at the C5 position of the respective Pyrimidine structure of either dU (replacing dT) or dC. This way, you can introduce a 3’ Alkyne-activation site without blocking the 3´OH end.