(Spanish National Centre for Biotechnology (CNB-CSIC)
Designing your sgRNA is a key step towards a successful CRISPR-Cas genome editing experiment. There a number of online tools dedicated to designing sgRNAs. One of the latest and most complete tool is Breaking Cas, which enables users to design sgRNAs using from all the genomes available on the ENSEMBL database.
For first-time users, you can read our guide here
To find out more about CRISPR-Cas, visit our CRISPR-Cas page!