MBQ-1 (Figure 1) stands for metabion Black Quencher 1
and it consists of a dark quencher often used in dual-labelled probes for real-time PCR.
Dark Quenchers absorb energy without emitting fluorescence. They are in fact non-fluorescent chromophores.
The MBQ-1 dark quencher is structurally identical to its respective BHQ-counterpart and exhibits strong absorption between 480 nm and 580 nm, making it highly effective at quenching fluorophores that fluoresce within this range, such as 6-Fam, TET, JOE, HEX, or a variety of Atto dyes.
The absorbance spectra of MBQ-1 is identical to the absorbance spectra of BHQ-1 (Figure 2), which indicates that also the quenching range is identical.
For more details, please refer to our Dual-Labelled Probes (DLPs) and the corresponding Reporter-Quencher combination table.
MBQ-1 can quench fluorescence through both FRET and static quenching mechanisms.
Figure 1. Structure of 3'-MBQ-1.
Figure 2. Absorbance spectra of BHQ-1- and MBQ-1-labelled oligonucleotides. As visible, the absorbance max of MBQ-1 is 534 nm, exactly as its counterpart BHQ-1.
metabion offers MBQ-1 at 3' in its DNA and RNA dual-labelled probes portfolio: