MBQ-2 (Figure 1) stands for metabion Black Quencher 2
and it consists of a dark quencher often used in dual-labelled probes for real-time PCR.
Dark Quenchers absorb energy without emitting fluorescence. They are in fact non-fluorescent chromophores.
The MBQ-2 dark quencher is structurally identical to its respective BHQ-counterpart and exhibits strong absorption between 550 nm and
650 nm, making it highly effective at quenching fluorophores that
fluoresce within this range, such as Tamra, ROX, or a variety
of Atto dyes.
The absorbance spectra of MBQ-2 is identical to the absorbance spectra of BHQ-2 (Figure 2), which indicates that also the quenching range is identical.
For more details, please refer to our Dual-Labelled Probes (DLPs) and the corresponding Reporter-Quencher combination table.
MBQ-2 can quench fluorescence through both FRET and static quenching mechanisms.
Figure 1. Structure of 3'-MBQ-2.
Figure 2. Absorbance spectra of BHQ-2- and MBQ-2-labelled oligonucleotides. As visible, the absorbance max of MBQ-2 is 579 nm, exactly as its counterpart BHQ-2.
metabion offers MBQ-2 at 3' in its DNA and RNA dual-labelled probes portfolio: