Digoxigenin-labelled probes are commonly used in in situ hybridization, Northern and Southern blot. Following hybridization to their targets, Dig-labelled probes can be detected using anti-Dig antibodies labelled with dyes or enzymes.
Metabion offers Digoxigenin in its DNA and RNA portfolio. Click here
|MW 5'||724 g/mol|
|MW 3'||754 g/mol|
|Extinction coefficient (260 nm)||13 mM-1cm-1|
Hauptmann, G., Gerster, T..Two-color whole-mount in situ hybridixation to vertebrate and Drosophila embryos.Trends Genet. (1994), 10: 266.