5-Me-dC stands for 5-methyl deoxycytosine. When inserted in an oligonucleotide, 5-Me-dC increases the stability of the resulting duplex by raising the Tm of 1.3°C per 5-Me-dC residue added. Therefore, 5-Me-dC- modified oligos can hybridize more efficiently to their targets. Use of 5-Me-dC results in:
- Strong-binding PCR primers
- Reduction of inflammatory response when used in antisense oligos rich in CpG motives.
- Allows DNA methylation studies.
Metabion offers 5-Met-dC in its DNA and RNA portfolio. Click here
|Extinction coefficient (260 nm)||5.7 mM-1cm-1|
Y. Lebedev, N. Akopyants, T. Azhikina, Y. Shevchenko, V. Potapov, D. Stecenko, D. Berg, E. Sverdlov. Oligonucleotides containing 2-aminoadenine and 5-methylcytosine are more effective as primers for PCR amplification than their nonmodified counterparts. Genet Anal. (1996), 13: 15-21.
S.P. Henry, K. Stecker, D. Brooks, D. Monteith, B. Conklin, C.F. Bennett. Chemically modified oligonucleotides exhibit decreased immune stimulation in mice. J. Pharmacol. Exp. Ther. (2000), 292: 468-479.
T. Ishii, M. Fujishiro, M. Masuda, S. Teramoto, T. Matsuse. A methylated oligonucleotide induced methylation of GSTP1 promoter and suppressed its expression in A549 lung adenocarcinoma cells. Cancer Letters (2004), 212: 211-223.