6-Fam

6-Fam (6-carboxyfluorescein) is one single isomer of the dye Fluorescein. It is usually coupled to DNA and RNA oligonucleotides via an amide bond and it has a good solubility in water. Spectral characteristics of 6-Fam are identical to those of FITC (Fluorescein-5,6-isothiocyanate), which consists of a reactive specie of fluorescein bearing an isothiocyanate reactive group. 6-Fam can be successfully used with most fluorescent detection devices available in the market.

Fluorescent 6 Fam

Metabion offers 6-Fam modifications in its DNA and RNA portfolio. Click here

6-Fam as 5' modification
MW  538 g/mol
Excitation maximum 495 nm
Emission maximum 520 nm
Extinction coefficient (260nm) 20.9 mM-1cm-1
6-Fam as 3' modification
MW  598 g/mol
Excitation maximum 495 nm
Emission maximum 520 nm
Extinction coefficient (260nm) 20.9 mM-1cm-1
6-Fam as internal 6-Fam-dT
MW  852 g/mol
Excitation maximum 495 nm
Emission maximum 520 nm
Extinction coefficient (260nm) 30.2 mM-1cm-1

 

K.J. Livak, S.J.A. Flood, J. Marmaro, W. Giusti, and K. Deetz. Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization. PCR Methods Appl. (1995), 4: 1-6

 

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