2’ OMe-A is a ribonucleotide that can be inserted in oligonucleotides to increase their Tm. Additionally, 2’ OMe-A protects the oligonucleotide against endonuclease cleavage, although NOT against exonuclease digestion. Therefore, it is particularly useful as a modification in antisense oligonucleotides, which will have their Tm increased by 1.3°C per 2’ OMe-A added. However, metabion recommends to additionally phosphorothiolate such 2’ OMe-A-antisense, in order to protect them against exonuclease degradation. To learn more about PTO modification, click here.
Metabion offers the modification in its DNA and RNA portfolio. Click here
|Extinction coefficient (260 nm)||13.7 mM-1cm-1|
P.M. Brett, J.F. Johnston, H. Sasmor and L.L. Cummins. Nuclease Resistance and Antisense Activity of Modified Oligonucleotides Targeted to Ha-ras. JBC (1996), 271: 14533-14540.