RNA qPCR probes

Metabion's RNA dual labelled probes (RNA DLPs) are optimized for use in real-time quantitative PCR. Moreover, our RNA DLPs are produced according to technologically advanced synthesis and purification protocols, to ensure you that the eventual background noise will be reduced to an absolute minimum.

Our range of reporter-quencher combinations suit probe-hydrolysis as well as probe-hybridisation based assays. We believe this will cover your needs for single or multiplex real-time PCR applications.

We offer our RNA DLPs in final yields in nmoles. With this, we introduce a transparent and best price-performance scheme for our broad selection of dual labelled probes as follows:

  • 5 scales based on delivered quantities tailored to your routine needs (see our Portfolio).
  • Length independent guaranteed (range) of delivered quantities in nmoles for probes from 15 to 40mers. Please note that OD260 values are a measure of total nucleotides´ optical density. Hence, neither purity nor amount of ordered substance are clearly reflected. Let’s walk through a relatively simple example:

    1 OD of the 20mer 5´CAT CGT ATT CGA TGC TAC GT 3´
    translates into approximately 5 nmol.

    1 OD of the 40mer 5´CAT CGT ATT CGA TGC TAC GT CAT CGT ATT CGA TGC TAC GT 3´
    translates into approximately 2.5 nmol.

    Therefore, a 1 OD guaranteed amount of delivered product can vary significantly, while metabion´s commitment to delivered yields in nmol for probes ranging from 15 to 40 nucleotides does not allow for ambiguity in terms of what you expect and pay for.

  • metabion´s routine dual labeled probe portfolio perfectly covers the spectrum of applicable excitation and detection wavelengths, with reporter-quencher combinations that are suitable for all commonly used real-time PCR platforms. However, do not hesitate to ask for non-listed combinations including internally or multi-labeled oligos and probes. Contact us
  • Our Prices include HPLC purification as well as Mass-Check QC to ensure you receive the highest-quality product possible.
  • You will receive your RNA DLP dry, unless otherwise specified.

It matters what you actually get for your money!

Any question? Visit our FAQs page or contact us!

Our standard custom RNA portfolio features

5 yield ranges based on length independent delivered quantities from 1-50 nmol for probes from 15-40 nucleotides.

  • ≥   1 <   5 nmol
  • ≥   5 < 10 nmol
  • ≥ 10 < 20 nmol
  • ≥ 20 < 30 nmol
  • ≥ 30 < 50 nmol

Reporter-Quencher Combinations

3' Quencher
  BHQ®-1 BHQ®-2 BBQ®-650 Eclipse® Dabcyl    
Abs range (nm) 480-580 550-650 550-750 390-625 380-550    
Abs max (nm) 535 579 650 522 453    
5' Reporter Possible combinations Abs (nm) Em (nm)
LC® CYAN500     450 500
6-Fam   495 520
Fluo   495 520
FITC   490 525
Tet   521 536
Joe     522 548
Yakima Yellow®     530 549
Hex 535 556
Cy3     546 563
Tamra   564 579
Rox     576 601
Texas Red®     586 610
LC® Red 610     590 610
LC® Red 640       625 640
Cy5       646 662
Cy5.5       683 705
IRD®700       685 705
5' Quencher
  BHQ®-1 BHQ®-2 BBQ®-650 Eclipse® Dabcyl    
Abs range (nm) 480-580 550-650 550-750 390-625 380-550    
Abs max (nm) 535 579 650 522 453    
3' Reporter Possible combinations Abs (nm) Em (nm)
6-Fam   495 520
Fluo   495 520
Joe     522 548
Hex     535 556
Cy3         546 563
Rox     576 601
LC® Red 610     590 610
LC® Red 640       625 640
Cy5       646 662
Cy5.5       683 705

All our DLP probes are HPLC purified and mass-checked for best quality assurance!

RNA Portfolio price list
download PDF

Order Forms

RNA Dual Labeled Probes  download xls
Also available in our web order system (WOP)

RNA Guidelines

Guidelines for Handling RNA download PDF

Credit Card Payment Form

Credit Card Payment Form download PDF

Price List

RNA Portfolio price list
download PDF