qPCR Master Mixes

We offer high quality ready-to-use premixes for real-time applications -

  • suitable for most real-time/qPCR platforms
  • suitable for (1) intercalating dye- or (2) probe-based qPCR applications
  • suitable for HRM analysis (EvaGreen Master Mixes)
  • with or w/o ROX reference
  • with or w/o UNG carry-over prevention
  • including optimized hot-start polymerase
  • available in two packaging sizes S and L
  • customized special packaging or quantities upon request
  • Do you need customized solutions (lyophilized, prefilled) or bulk amounts? Please tell us your needs for preparing a special quote and mailto: customerservice@metabion.com

metabion, the expert in custom synthesis of primers and probes, now offers 2x ready-to-use real-time PCR hot start mixes using either green-fluorescent DNA stain or labeled DNA probes. The reaction chemistry of the mixes is optimized for block-based PCR instruments (no glass capillaries) and suitable for most qPCR machines and platforms (+/- ROX reference).

Master mixes contain all reagents (except template and oligos) required for qPCR in a premixed 2x concentrated ready-to-use solution. High specificity and sensitivity of the mixes are achieved by an optimized hot-start polymerase. Its activity is blocked at ambient temperature and switched on automatically at the onset of the initial denaturation. The thermal activation prevents the extension of nonspecifically annealed primers and primer-dimer formations at low temperatures during PCR setup.

The mixes contain dUTP instead of dTTP, in order to eliminate carry-over contamination of DNA from previous PCR reactions by the use of UNG (Uracil-N-Glycosylase).

UNG (Uracil-N-Glycosylase)
The UNG treatment at the onset of thermal cycling removes Uracil residues from dU-containing DNA of previous PCR amplifications preventing it from serving as template (carry-over contamination control).

ROX passive reference dye
ROX is a passive reference dye and does not take part in the PCR reaction. However, it allows to normalize for non-PCR related signal variation and provides a baseline in multiplex reactions.

(1) Intercalating Dye qPCR Solutions:

EvaGreen® Master Mixes

mi-real-time EvaGreen® Master mixes (2x conc.) are designed for the quantitative real-time analysis of DNA samples using the fluorescent DNA stain EvaGreen®. The fluorescent dye in the master mix intercalates into the amplification product during the PCR process enabling for rapid analysis of target DNA. No need for sequence-specific labeled probes. It provides an easy-to-handle and powerful tool for quantification of sample DNA in a broad dynamic range of up to 6 orders of magnitude with exceptional sensitivity and precision.

EvaGreen® is superior to the widely used SYBR®Green and is suitable for precise HRM analysis (High resolution melting).

realtime curve

(2) Probe-Based qPCR Solutions:

Probe Master Mixes

mi-Real-Time Probe Master Mixes (2x conc.) are designed for the quantitative real-time analysis of DNA samples using DNA probe-based detection. Master Mixes are recommended for 5´Nuclease- or FRET Hybridization Probe-assays using fluorescent probes. Being the experts in custom synthesis of primers and probes for real-time PCR, we now also offer Probe Master Mixes as valuable high quality “accessories” for the successful performance of your assays.

EvaGreen® Master mixes 
Product Cat. No. Quantity
mi-real-time EvaGreen® Master mi-E2001S
mi-E2001L
100 rx (50 µl)
500 rx (50 µl)
mi-real-time EvaGreen® Master (ROX+) mi-E2002S
mi-E2002L
100 rx (50 µl)
500 rx (50 µl)
mi-real-time EvaGreen® Master (UNG+) mi-E2003S
mi-E2003L
100 rx (50 µl)
500 rx (50 µl)
mi-real-time EvaGreen® Master (UNG+/ROX+) mi-E2004S
mi-E2004L
100 rx (50 µl)
500 rx (50 µl)

 

Probe Master mixes 
Product Cat. No. Quantity
mi-real-time Probe Master mi-E2005S
mi-E2005L
100 rx (50 µl)
500 rx (50 µl)
mi-real-time Probe Master (ROX+) mi-E2006S
mi-E2006L
100 rx (50 µl)
500 rx (50 µl)
mi-real-time Probe Master (UNG+) mi-E2007S
mi-E2007L
100 rx (50 µl)
500 rx (50 µl)
mi-real-time Probe Master (UNG+/ROX+) mi-E2008S
mi-E2008L
100 rx (50 µl)
500 rx (50 µl)

Do you need customized solutions (lyophilized, prefilled) or bulk amounts? Please tell us your needs for preparing a special quote and mailto: customerservice@metabion.com

ProductDatasheetsMSDS
mi-real-time EvaGreen® Master mi-E2001S
mi-E2001L
MSDS
MSDS
mi-real-time EvaGreen® Master (ROX+) mi-E2002S
mi-E2002L
MSDS
MSDS
mi-real-time EvaGreen® Master (UNG+) mi-E2003S
mi-E2003L
MSDS
MSDS
mi-real-time EvaGreen® Master (UNG+/ROX+) mi-E2004S
mi-E2004L
MSDS
MSDS
mi-real-time Probe Master mi-E2005S
mi-E2005L
MSDS
MSDS
mi-real-time Probe Master (ROX+) mi-E2006S
mi-E2006L
MSDS
MSDS
mi-real-time Probe Master (UNG+) mi-E2007S
mi-E2007L
MSDS
MSDS
mi-real-time Probe Master (UNG+/ROX+) mi-E2008S
mi-E2008L
MSDS
MSDS

How to order

Online

Preferred ordering mode is using our web order portal for most convenient web shopping!

Email

send an email to mi@mymetabion.com, include your customer id (if you have one).
If you are a new customer, please additionally provide us with

  • Your shipping and billing address
  • Any other information like Purchase Order number, VAT number (VAT only for customers resident in the EU) etc
  • and any special shipping requests.

Confirmation

Orders which indicate an email address will be confirmed by email.

Delivery

Our default shipping mode is sending by Express service overnight at EURO 10,00 per shipment within Germany. If the value of your order is > EURO 250,00, shipping within Germany is free of charge!

For other countries please see our shipping table.

Note that Shipments on blue ice incur an additional charge of 4.50 EUR.
Shipments on dry ice incur an additional charge of 9.50 EUR.

Master mixes will be shipped as soon as possible after receiving your confirmed order. If you want to combine shipments of different product categories/orders for cost saving reasons, please indicate.

To assure the quality of our enzymes, they are shipped on cool packs within Germany and Europe. If longer travelling times are expected (> 3 days, customs, etc.) dry ice can be used instead.

Transportation damages have to be immediately/ upon receipt of goods notified to the parcel service!

All our products are offered according to our terms & conditions. For in vitro use and research purposes only!