back to overview

Do I need to have my dsDNA/duplex DNA purified?

This depends on the complexity (length, base composition, modifications) of your requested molecule, as well as on the application desired. Failure sequences may be generated both during and post-synthesis. Due to the nature of synthesis chemistry (coupling efficiency < 100%) and/or post-synthetic modification procedures, there will remain failure sequences (n-x), free modifiers and non-labeled products, respectively, in the "crude" unpurified product.

High pressure liquid chromatography (HPLC) purification is standard (no additional charge) for dsDNA/duplex DNA. For applications in cells and in vivo, we recommend an additional Size-Exclusion Chromatography (SEC). This is a size exclusion chromatography purification that removes salts and other small molecules, whose quantity and/ or quality can be toxic for cells (please inquire).