How are click-oligos made?
Additionally, an alkyne site is introduced internally or at the 5’/3’ end in the following fashion:
- via an Alxyl moiety, which is sequence independent and allows the addition of an Alkyne group via a C6 (hexynyl)-linker to the phosphate of the 5´ nucleoside;
- via an Alrol moiety, which is sequence independent and allows the addition of an Alkyne group via a serinol-linker to the 3’ OH of the 3´ nucleoside;
- via replacing a C or T at any position of your DNA or RNA oligonucleotide (internally or at the 5’/3’ end) with AldC/AldU, respectively (sequence dependent). AldU and AldC carry a C8-Alkyne motif at the C5 position of the respective Pyrimidine structure of either dU (replacing dT) or dC.
“Ready-to-use/fully labeled” custom oligonucleotides are coupled to the Azide modifier of your choice (please refer to our product portfolio for a full list of our Azide modifiers to use in the “click reaction” described by Carell and colleagues.