How are nGS oligos made?
metabion produces nGS oligos in a dedicated synthesis pipeline. This dramatically minimizes the risk of cross-contamination and guarantees an unmatched degree of high quality. With our nGS oligos, you can perform your Next Generation sequencing experiments with the peace of mind that you work with reagents you can trust.
Oligos are made using a DNA synthesizer which is basically a computer-controlled reagent delivery system. The first base is attached to a solid support, usually a glass or polystyrene bead, which is designed to anchor the growing DNA chain in the reaction column. DNA synthesis consists of a series of chemical reactions.
Each cycle of reactions results in the addition of a single DNA base. A chain of DNA bases can be built by repeating the synthesis cycles until the desired length is achieved.