What concentration of ZNA® primers and probes are recommended for PCR applications?
ZNA®s display high sensitivity in PCR applications, due to enhanced kinetics in target recognition and “scanning”. This is the reason why ZNA® primers and probes should be used in lower concentration, compared to other DNA-oligos. Typical concentrations are 100-200 nM for primers and 200 nM for probes. A concentration even lower than 50 nM have been proven successful in PCR, without lost of sensitivity. This may represent a great advantage in multiplex assays, which use multiple sets of primers.