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What do I resuspend my RNA DLP in and what concentration should I aim to?

Distilled sterile water pretreated with DEPC, or any nuclease free biological buffer (i.e. with physiological pH) are acceptable as diluents. The recommended diluent volume is 100 µl - 1 ml, the concentration depending on the application to be used and the yield of the resulting product (see also FAQ "How stable is my RNA DLP once I have resuspended it?").

Please see also our RNA-handling guidelines!