What is the maximum length a RNA DLP can be synthesized?

Using our optimized production pipeline, we can deliver RNA DLPs of over 40 bases. The maximum length of an oligonucleotide depends both on the sequence and on the modifications of interest. For example, consider a DLP having a reporter at the 5’-end and a quencher at the 3’-end. The length of the DLP can compromise the activity of the quencher, if the distance between reporter and quencher is too high. Therefore, we consider a length of 40 bases as maximum, for a DLP probe to function optimally.

To get best performance for your experiments, we can help you designing your probe. Please do not hesitate to contact us!