What kind of documentation do I get with my RNA oligos?
The label on the oligo tube shows basic information like oligo name, name of person who ordered, oligo sequence including modifications, oligo ID, amount of RNA (OD260 and nmol), Tm, and molecular weight.
In addition, you will receive a synthesis report containing more detailed information on the physical-chemical properties of the oligo, such as base composition, base count, purification grade, amount of RNA (OD260 and nmol), Tm and molecular weight.
Each and every oligo produced and delivered is analyzed by either MALDI- or ESI-ToF before release, and Mass-Check documentation/spectra will be provided upon additional charges (desalted RNA) or free of charge (HPLC purified RNA). The following terminology is used for differentiating between offered QC:
For desalted RNA:
Mass Check
Standard quality control performed on each and every oligo. Either MALDI- or ESI-ToF, subject to the "nature of the oligo", and metabion internal procedures. This service is free of charge and no printed/pdf documentation is provided.
MALDI-ToF
Explicitly ordered and performed MALDI-ToF check. Product delivered with MALDI-ToF spectra. Additional charges apply.
ESI-ToF
Explicitly ordered and performed ESI-ToF check. Product delivered with ESI-ToF spectra. Additional charges apply.
Analytical HPLC
Performed Mass Check and Analytical HPLC (see FAQ "What is the difference between preparative and analytical HPLC?"). Product delivered with analytical HPLC but without MS spectra. Additional charges apply.
Mass Check + Analytical HPLC
Explicitly ordered and performed Mass Check (MALDI-ToF or ESI-ToF subject to the "nature of the oligo" and metabion internal procedures) and Analytical HPLC (see FAQ "What is the difference between preparative and analytical HPLC?"). Product delivered with analytical HPLC and MS spectra. Additional charges apply.
For HPLC purified RNA:
Mass Check
Standard quality control performed on each and every oligo. Either MALDI- or ESI-ToF, subject to the "nature of the oligo", and metabion internal procedures. This service is free of charge and will always be delivered with hard copies of the Mass Check spectrum for all our HPLC purified RNA products.
Analytical HPLC
Mass Check (MALDI-ToF or ESI-ToF subject to the "nature of the oligo" and metabion internal procedures) and explicitly ordered Analytical HPLC (see FAQ "What is the difference between preparative and analytical HPLC?"). Product delivered with analytical HPLC and MS spectra. Additional charges apply.