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Biocalculator

OligoCalc is a user-friendly online tool for calculating properties of DNA and RNA strands, including molecular weight, melting temperature, and self-complementarity.

It's widely used and accessible since 1997 (Figure 1).

OligoCalc is available at http://biotools.nubic.northwestern.edu/OligoCalc.html.


Figure 1. Entry and main calculation screen for OligoCalc.



References

Kibbe WA. OligoCalc: an online oligonucleotide properties calculator. Nucleic Acids Res. 2007 Jul;35(Web Server issue):W43-6.