2’-OMe-U (Figure 1) is a ribonucleotide that can be inserted in oligonucleotides to increase their Tm. Additionally, 2’-OMe-U protects the oligonucleotide against endonuclease cleavage, although NOT against exonuclease digestion. Therefore, it is particularly useful as a modification in antisense oligonucleotides, which will have their Tm increased by 1.3 °C per 2’-OMe-U added. However, metabion recommends to additionally phosphorothiolate such 2’-OMe-U-antisenses, in order to protect them against exonuclease degradation. To learn more about PTO modification, click here.
Figure 1. Structure of 2’-OMe-U (R = sequence).
metabion offers the modification in its DNA and RNA portfolio: