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5-hydroxymethylcytosine (5-Hydroxymethyl-C, Figure 1) is also known as “the sixth base”, given that it has been detected in all animal tissues, with particularly high level in promoter and intragenic regions. 5-hydroxymethylcytosine derives from 5-mC, which plays an important role as an epigenetic marker in gene expression and regulation. However, which role 5-hydroxymethylcytosine plays is still not clear. Incorporation of this base in synthetic oligonucleotides will help researchers elucidating the role of this base.

Figure 1. Structure of 5-hydroxymethylcytosine (R = sequence).

metabion offers 5-Hydroxymethyl-C in its DNA portfolio: