ATTO 495 is an innovative fluorescent label based on the well-known Acridine Orange dye. It is characterized by strong absorption, a high fluorescence quantum yield, and excellent thermal and photostability. In solid matrices or at low temperatures, the dye exhibits intense and long-lasting phosphorescence. ATTO 495 is moderately hydrophilic, and its fluorescence is efficiently excited within the 465-510 nm wavelength range.
Atto 495 (Figure 1) can be used as an alternative of Alexa Fluor®488.

Figure 1. Structure of 5'-Atto 495 (R = sequence).
metabion offers the Atto 495 modification in its DNA and RNA portfolio (as a 5'-, 3'- or internal Atto 495-dT modification):