ATTO 565 is a fluorescent label from the rhodamine dye family, known for its strong absorption, high fluorescence quantum yield, and excellent thermal and photostability. This makes ATTO 565 ideal for single-molecule detection, flow cytometry (FACS), fluorescence in situ hybridization (FISH), and more. The dye is moderately hydrophilic and is most efficiently excited in the 545 - 575 nm range.
Atto 565 (Figure 1) can be used as an alternative to Alexa Fluor 568 or Alexa Fluor 546.
Figure 1. Structure of 5'-Atto 565 (R = sequence).
metabion offers Atto 565 in its DNA and RNA portfolio (as a 5'-, 3'- or internal Atto 565-dT modification):