ATTO Rho13 is a fluorescent dye from the rhodamine family. It is characterized by strong absorption, high fluorescence quantum yield, and excellent thermal and photostability. As a cationic dye, it carries a net positive charge of +1 after binding to a substrate. The dye is moderately hydrophilic and its fluorescence is optimally excited within the 545 - 575 nm wavelength range.
Atto Rho13 (Figure 1) can be used as an alternative of Alexa Fluor®594 or Alexa Fluor®610.
Figure 1. Structure of 5'-Atto Rho13 (R = sequence).
metabion offers the Atto Rho13 modification in its DNA and RNA portfolio (as a 5'-, 3'- or internal Atto Rho13-dT modification):