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Biotin can be used as an oligonucleotide modification for a variety of assays. Moieties or surfaces conjugated with streptavidin will bind Biotin-labelled oligos tightly. Additionally, the TEG spacer minimizes steric hindrance between the Biotin moiety and the oligonucleotide (Figure 1). This, for example, facilitates the capture of Biotin-labelled oligos onto streptavidin coated surfaces.

Figure 1. Structure of 5'-Biotin-TEG (R = sequence).

metabion offers Biotin-TEG as a 5'-, 3'- or internal modification in its standard DNA and RNA portfolio: