Bromo-dU

5-Bromo-deoxyuridine (Bromo-dU, Figure 1) is a halogenated nucleotide, commonly used in X-ray crystallography to facilitate the determination of DNA structure. Additionally, bromo nucleotides are photo-labile and therefore suitable for UV-crosslinking experiments to study protein-DNA complexes. 5-Br-dU was also used to create photo-aptamers able to crosslink with their target.

Figure 1. Structure of 5-Bromo-deoxyuridine (R = sequence).

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References

1. Hendrickson WA., Ogata CM. [28] Phase determination from multiwavelength anomalous diffraction measurements. Methods Enzymol. 1997;276:494-523.