Cyanine 5 (Cy5, Figure 1) can be efficiently used in multiplex PCR in combinations. Cy5 is a fluorescent dye that belongs to the Cyanine family of synthetic polymethine dyes and it represents an excellent alternative to WellRED dye D4 and LIZ.
Figure 1. Structure of 5'-Cyanine 5 (R = sequence).
metabion offers the Cy5 modification in its DNA and RNA portfolio: