Eclipse® (Figure 1) is a dark quencher often used in dual-labelled probes. As dark quenchers, Eclipse® absorbs energy without emitting fluorescence. It is in fact a non-fluorescent chromophore.
Figure 1. Structure of 5'-Eclipse® (R = sequence).
metabion offers Eclipse® in its DNA and RNA dual-labelled probes portfolio. Also, Eclipse® is our standard quencher for MGB probes (Click here). It offers higher sensitivity in real-time PCR probes due to lower background fluorescence and quenches a broad dye emission spectra range.