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Fluorescein (resorcinolphthalein, Figure 1) is a fluorescent dye consisting of two isomers, 5(6)-carboxyfluorescein, linked to the spirolactam ring system, one of which is 6-Fam (6-carboxyfluorescein), a most commonly used dye; it is most efficiently quenched by BHQ-1.

Figure 1. Structure of 5'-Fluorescein (R = sequence).

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