IRDye®700 (Figure 1) is a near-infrared fluorescent dye commonly used to label DNA and RNA primers and probes. IRDye®700 is characterized by low-background autofluorescence, which ensures good detection sensitivity and offers the opportunity to use IRDye®700-labelled oligonucleotides in a variety of applications, both in vivo and in vitro. IRDyes are ideally suited for concurrent multi-colour imaging applications by virtue of the dyes' narrow emission spectrum and the absence of crossover between fluorophores. IRDye®700 represents an excellent alternative to WellRED dye D3.
Figure 1. Structure of 5'-IRDye®700 (R = sequence).
metabion offers IRDye®700 as a 5'-modification in its DNA and RNA portfolio: