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The chemical name of Rox (Figure 1) is 6-Carboxyl-X-Rhodamine. It is an inert fluorescent dye which is normally used as a reference dye to normalize the fluorescence signal arising from fluorescent probes in real-time PCR applications, as its fluorescence signal is not affected by reaction in PCR.

Rox represents an excellent alternative to PET®, due to their similar spectral properties.

Figure 1. Structure of 5'-Rox (R = sequence).

metabion offers the Rox modification in its DNA and RNA portfolio:

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