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Breaking Cas

Breaking Cas enables users to design sgRNAs using from all the genomes available on the ENSEMBL database.

Spanish National Biotechnology Centre (Centro Nacional de Biotecnología, CNB - Consejo Superior de Investigaciones Científicas, CSIC)

Designing your sgRNA is a key step towards a successful CRISPR-Cas genome editing experiment. There a number of online tools dedicated to designing sgRNAs. One of the latest and most complete tool is Breaking Cas.

For first-time users, you can read our guide here.

To find out more about CRISPR-Cas, visit our CRISPR-Cas page!


Oliveros JC., Franch M., Tabas-Madrid D., San-León D., Montoliu L., Cubas P., Pazos F. Breaking-Cas-interactive design of guide RNAs for CRISPR-Cas experiments for ENSEMBL genomes. Nucleic Acids Res. 2016 Jul 8;44(W1):W267-71.