We developed our patented high-throughput technology platform in order to produce high quality oligos in 96well microtiter plate format for all applications that require large number of oligos (including a wide variety of plate types, normalization and mixing/pooling).
The “classic” definition of High Throughput Oligos goes back to the need for large numbers of mainly unmodified, moderately long standard oligos delivered in microtiter plate formats to suit the requirements of increasingly popular Genomic screening/microarray programs.
From the beginning, metabion recognized the risk of compromising quality coming along with high-throughput production of oligonucleotides. Hence, we were determined to prove, that our slogan “quantity meets quality” comes alive by developing our own high throughput technology platform to produce HTOs.
“Heart” of this platform is our inhouse developed 96-Parallel Synthesizer, called BH96 (“Blue Hedgehog” 96), which gives us the chance to produce high throughput-high quality oligos, a unique feature we are very proud to build on. The uniqueness of our BH96 is convincingly reflected by the fact that metabion could protect the instrument by patent! As quality was, is, and will always be metabion´s first priority, the “kidney” of our HTO line is Sequenom´s MassArray System, which enables us to check each and every oligo produced by Maldi-ToF. With the help of this specially adapted system, we can “filter” our synthesis products very quickly for potential quality nonconformities referring to our stringently applied quality criteria. Non-conform oligos are immediately re-synthesized so that only completely checked and approved plates are released.
Our outstanding performance in terms of High Throughput Oligos synthesis is based on:
- Quantity delivered: we guarantee delivery amounts based on final yields in nmol, rather than on abstract OD260 values;
- Quality guarantee: metabion has been the first company in Europe to introduce 100% quality check by Maldi-ToF, and yet being able to offer oligos in Microtiter Plates (MTPs) at best value prices.
Our commitment to quality is further emphasized by our production procedures having been ISO certified according to DIN EN 9001 and 13485 since 2001 and 2010, respectively.
Our standard custom DNA High Throughput Oligos - Microtiter Plate format - Portfolio
Delivery in 96-well MTPs:
- 1.2 ml round well/U-bottom
- 1.0 ml “Matrix” microplates w/o barcode
- 1.0 ml “Matrix” microplates 2D barcoded
3 yield ranges based on length-independent delivered quantities from 11-80 nts:
- ≥ 10 < 25 nmol
- ≥ 25 < 50 nmol
- ≥ 50 < 100 nmol
We can also ship your oligos wet at the following concentrations:
For HPLC-purified oligos in MTP format, please inquire.
FAQ – you ask, we answer
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There are two ways of ordering:
- The preferred way is order transmission through our Web Order Portal for most convenient online shopping (please refer here for further details).
- You can order by sending us an e-mail at oligo@metabion.com with our pre-formatted excel order file as attachment. Download respective Order Form.
When you write your e-mail, please make sure to include the following required information in the excel template:
- Name of the oligo/s.
- Sequence of the oligo/s in 5’-3’ orientation.
- Yield range.
- Position of the oligo.
- Delivery form (dry/in water/buffer + concentration).
- Modifications (2'-O-Methyl RNA and 5-Methyl-dC can be selected here).
If you are a new customer, please additionally provide us with:
- Your shipping and billing address.
- Any other information like Purchase Order number, VAT number (VAT only for customers resident in the EU), etc.
In case you choose to transmit orders via e-mail using your own format(s), we need to alert you that above mentioned information in bold print is obligatory for processing your order. Due to extra efforts necessary for individual order format transfer into our system, order processing will take longer as compared to preferred web orders and pre-formatted e-mails. Please note that only files updated to the latest excel/word version (e.g. .xlsx or .docx) are accepted.
How can you convert files to newer formats?
Open
the file in a recent Office program and save the file in a newer format.
For this go to “File” → “Save as” and choose the newer format from the
“Save as type” dropdown menu.
Jump on our Web Order Portal, look for the product category headline under “DNA Custom Synthesis Services” called “DNA High Throughput Oligos (HTOs)”, and open the respective order form and start filling it. Options provided are self-explanatory. The system shall guide you through the ordering process.
Place your order and benefit from our yield scale and pricing scheme.
If you want to connect your eProcurement System with our Web Order Portal (e.g. OCI - Open Catalog Interface), please simply contact our Customer Service (customerservice@metabion.com).
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Shipping condition dry or wet in 6 different normalization options free of charge:
- Standard desalted, “ready-to-use” and 100% QCed oligos at top quality.
- Minimum number of oligos of the same kind (scale, modification, normalization, shipping condition) is 24 per 96well plate. Combination of batches of >/=24 congeneric oligos in one plate is allowed (e.g. 4x24, 2x48, 3x24, 2x24/1x48 etc.).
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The label on the oligo plate shows basic information like name of person who ordered, name of the plate and order ID.
In addition, you will receive a synthesis report containing more detailed information, such as name of the oligos, physical-chemical properties of the oligos, such base composition, base count, purification grade, amount of DNA (OD260 and nmol), Tm and molecular weight.
While each and every oligo produced and delivered is characterized by either MALDI- or ESI-ToF before release, Mass-Check documentation/spectra will only be provided if requested at the time of order placement. Additional charges may apply. The following terminology is used for differentiating between offered QC options including respective documentation coverage in our order forms and on supporting documents delivered with the products:
Mass Check
Standard quality control performed on each and every oligo. Either MALDI- or ESI-ToF, subject to the "nature of the oligo", and metabion internal procedures. This service is free of charge and no printed/pdf documentation is provided.
MALDI-ToF
Explicitly ordered and performed MALDI-ToF check. Product delivered with MALDI-ToF spectra. Additional charges apply.
ESI-ToF
Explicitly ordered and performed ESI-ToF check. Product delivered with ESI-ToF spectra. Additional charges apply.
Mass Check + Analytical HPLC
Explicitly ordered and performed Mass Check (MALDI-ToF or ESI-ToF subject to the "nature of the oligo" and metabion internal procedures) and Analytical High Pressure Liquid Chromatography (see FAQ "What is the difference between preparative and analytical HPLC?"). Product delivered with analytical HPLC and MS spectra. Additional charges apply.
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Of course we can and we would be happy to do so! Please inquire for feasibility and a quotation; write us an email at info@metabion.com.
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The real answer lies in the resolution limit of the purification method and on the coupling efficiency of the DNA synthesizer. We can synthesize DNA oligos of 220 bases and obtain sufficient quantities by HPLC purification to perform successful gene construction. However, it should be remembered that the longer the oligo, the greater the chance of accumulated sequence errors.
Coupling efficiency is the factor that mainly affects the length of DNA that can be synthesized. Base composition and synthesis scales will also be contributing factors. For more information regarding coupling efficiency, please refer to the FAQ "What is coupling efficiency?".
Importantly, the probability of premature synthesis interruption increases dramatically for longer oligos, due to poor nucleotide-coupling efficiency. Moreover, the synthesis of special “long oligos” requires more raw material, such as amidites and solutions that need to be used in higher excess.
However, as we are very experienced in producing very long oligos, do not hesitate to discuss your projects with our specialists.
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Yes, they are as follows: